RNA

Part:BBa_K3001020:Design

Designed by: Sydnee Calhoun   Group: iGEM19_Lethbridge_HS   (2019-10-10)


Lwa crRNA targeting RNA Mango


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 44
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 44
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 44
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 44
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A direct repeat stem loop is added to allow the crRNA to interact with Cas13a Lwa. The target sequence is designed to only activate Cas13a Lwa when the target RNA is present.


Source

Designed by Laura Keffer-Wilkes based off of design by Munich 2017. Synthesized by IDT.

References